Mixed culture biotechnology and its versatility in dark fermentative hydrogen production.

Over the years, extensive research has gone into fermentative hydrogen production using pure and mixed cultures from waste biomass with promising results. However, for up-scaling of hydrogen production mixed cultures are more appropriate to overcome the operational difficulties such as a metabolic shift in response to environmental stress, and the need for a sterile environment. Mixed culture biotechnology (MCB) is a robust and stable alternative with efficient waste and wastewater treatment capacity along with co-generation of biohydrogen and platform chemicals. Mixed culture being a diverse group of bacteria with complex metabolic functions would offer a better response to the environmental variations encountered during biohydrogen production. The development of defined mixed cultures with desired functions would help to understand the microbial community dynamics and the keystone species for improved hydrogen production. This review aims to offer an overview of the application of MCB for biohydrogen production.

Electrochemical-based approaches for the treatment of pharmaceuticals and personal care products in wastewater.

In recent times, emerging contaminants (ECs) like pharmaceuticals and personal care products (PPCPs) in water and wastewater have become a major concern in the environment. Electrochemical treatment technologies proved to be more efficient to degrade or remove PPCPs present in the wastewater. Electrochemical treatment technologies have been the subject of intense research for the past few years. Attention has been given to electro-oxidation and electro-coagulation by industries and researchers, indicating their potential to remediate PPCPs and mineralization of organic and inorganic contaminants present in wastewater. However, difficulties arise in the successful operation of scaled-up systems. Hence, researchers have identified the need to integrate electrochemical technology with other treatment technologies, particularly advanced oxidation processes (AOPs). Integration of technologies addresses the limitation of indiviual technologies. The major drawbacks like formation of undesired or toxic intermediates, s, energy expenses, and process efficacy influenced by the type of wastewater etc., can be reduced in the combined processes. The review discusses the integration of electrochemical technology with various AOPs, like photo-Fenton, ozonation, UV/H2O2, O3/UV/H2O2, etc., as an efficient way to generate powerful radicals and augment the degradation of organic and inorganic pollutants. The processes are targeted for PPCPs such as ibuprofen, paracetamol, polyparaben and carbamezapine. The discussion concerns itself with the various advantages/disadvantages, reaction mechanisms, factors involved, and cost estimation of the individual and integrated technologies. The synergistic effect of the integrated technology is discussed in detail and remarks concerning the prospects subject to the investigation are also stated.

Emerging trends and advances in valorization of lignocellulosic biomass to biofuels.

Sustainable technologies pave the way to address future energy demand by converting lignocellulosic biomass into fuels, carbon-neutral materials, and chemicals which might replace fossil fuels. Thermochemical and biochemical technologies are conventional methods that convert biomass into value-added products. To enhance biofuel production, the existing technologies should be upgraded using advanced processes. In this regard, the present review explores the advanced technologies of thermochemical processes such as plasma technology, hydrothermal treatment, microwave-based processing, microbial-catalyzed electrochemical systems, etc. Advanced biochemical technologies such as synthetic metabolic engineering and genomic engineering have led to the development of an effective strategy to produce biofuels. The microwave-plasma-based technique increases the biofuel conversion efficiency by 97% and the genetic engineering strains increase the sugar production by 40%, inferring that the advanced technologies enhances the efficiency. So understanding these processes leads to low-carbon technologies which can solve the global issues on energy security, the greenhouse gases emission, and global warming.

Lignocellulosic biomass-based glycoconjugates for diverse biotechnological applications.

Glycoconjugates are the ubiquitous components of mammalian cells, mainly synthesized by covalent bonds of carbohydrates to other biomolecules such as proteins and lipids, with a wide range of potential applications in novel vaccines, therapeutic peptides and antibodies (Ab). Considering the emerging developments in glycoscience, renewable production of glycoconjugates is of importance and lignocellulosic biomass (LCB) is a potential source of carbohydrates to produce synthetic glycoconjugates in a sustainable pathway. In this review, recent advances in glycobiology aiming on glycoconjugates production is presented together with the recent and cutting-edge advances in the therapeutic properties and application of glycoconjugates, including therapeutic glycoproteins, glycosaminoglycans (GAGs), and nutraceuticals, emphasizing the integral role of glycosylation in their function and efficacy. Special emphasis is given towards the potential exploration of carbon neutral feedstocks, in which LCB has an emerging role. Techniques for extraction and recovery of mono- and oligosaccharides from LCB are critically discussed and influence of the heterogeneous nature of the feedstocks and different methods for recovery of these sugars in the development of the customized glycoconjugates is explored. Although reports on the use of LCB for the production of glycoconjugates are scarce, this review sets clear that the potential of LCB as a source for the production of valuable glycoconjugates cannot be underestimated and encourages that future research should focus on refining the existing methodologies and exploring new approaches to fully realize the potential of LCB in glycoconjugate production.

Dark fermentative hydrogen production: Potential of food waste as future energy needs.

Globally, food waste (FW) is found to be one of the major constituents creating several hurdles in waste management. On the other hand, the energy crisis is increasing and the limited fossil fuel resources available are not sufficient for energy needed for emerging population. In this context, biohydrogen production approach through valorization of FW is emerging as one of the sustainable and eco-friendly options. The present review explores FW sources, characteristics, and dark fermentative production of hydrogen along with its efficiency. FW are highly biodegradable and rich in carbohydrates which can be efficiently utilized by anaerobic bacteria. Based on the composition of FW, several pretreatment methods can be adapted to improve the bioavailability of the organics. By-products of dark fermentation are organic acids that can be integrated with several secondary bioprocesses. The versatility of secondary products is ranging from energy generation to biochemicals production. Integrated approaches facilitate in enhanced energy harvesting along with extended wastewater treatment. The review also discusses various parameters like pH, temperature, hydraulic retention time and nutrient supplementation to enhance the process efficiency of biohydrogen production. The application of solid-state fermentation (SSF) in dark fermentation improves the process efficiency. Dark fermentation as the key process for valorization and additional energy generating process can make FW the most suitable substrate for circular economy and waste based biorefinery.

Photocatalytic degradation of four emerging antibiotic contaminants and toxicity assessment in wastewater: A comprehensive study.

Excessive usage and unrestricted discharge of antibiotics in the environment lead to their accumulation in the ecosystem due to their highly stable and non-biodegradation nature. Photodegradation of four most consumed antibiotics such as amoxicillin, azithromycin, cefixime, and ciprofloxacin were studied using Cu2O-TiO2 nanotubes. Cytotoxicity evaluation of the native and transformed products was conducted on the RAW 264.7 cell lines. Photocatalyst loading (0.1-2.0 g/L), pH (5, 7 and 9), initial antibiotic load (50-1000 µg/mL) and cuprous oxide percentage (5, 10 and 20) were optimized for efficient photodegradation of antibiotics. Quenching experiments to evaluate the mechanism of photodegradation with hydroxyl and superoxide radicals were found the most reactive species of the selected antibiotics. Complete degradation of selected antibiotics was achieved in 90 min with 1.5 g/L of 10% Cu2O-TiO2 nanotubes with initial antibiotic concentration (100 µg/mL) at neutral pH of water matrix. The photocatalyst showed high chemical stability and reusability up to five consecutive cycles. Zeta potential studies confirms the high stability and activity of 10% C-TAC (Cuprous oxide doped Titanium dioxide nanotubes for Applied Catalysis) in the tested pH conditions. Photoluminescence and Electrochemical Impedance Spectroscopy data speculates that 10% C-TAC photocatalyst have efficient photoexcitation in the visible light for photodegradation of antibiotics samples. Inhibitory concentration (IC50) interpretation from the toxicity analysis of native antibiotics concluded that ciprofloxacin was the most toxic antibiotic among the selected antibiotics. Cytotoxicity percentage of transformed products showed r: -0.985, p: 0.01 (negative correlation) with the degradation percentage revealing the efficient degradation of selected antibiotics with no toxic by-products.

Sustainable production of biosurfactants via valorisation of industrial wastes as alternate feedstocks.

Globally, the rapid increase in the human population has given rise to a variety of industries, which have produced a variety of wastes. Due to their detrimental effects on both human and environmental health, pollutants from industry have taken centre stage among the various types of waste produced. The amount of waste produced has therefore increased the demand for effective waste management. In order to create valuable chemicals for sustainable waste management, trash must be viewed as valuable addition. One of the most environmentally beneficial and sustainable choices is to use garbage to make biosurfactants. The utilization of waste in the production of biosurfactant provides lower processing costs, higher availability of feedstock and environmental friendly product along with its characteristics. The current review focuses on the use of industrial wastes in the creation of sustainable biosurfactants and discusses how biosurfactants are categorized. Waste generation in the fruit industry, agro-based industries, as well as sugar-industry and dairy-based industries is documented. Each waste and wastewater are listed along with its benefits and drawbacks. This review places a strong emphasis on waste management, which has important implications for the bioeconomy. It also offers the most recent scientific literature on industrial waste, including information on the role of renewable feedstock for the production of biosurfactants, as well as the difficulties and unmet research needs in this area.

Value addition through biohydrogen production and integrated processes from hydrothermal pretreatment of lignocellulosic biomass.

Bioenergy production is the most sought-after topics at the crunch of energy demand, climate change and waste generation. In view of this, lignocellulosic biomass (LCB) rich in complex organic content has the potential to produce bioenergy in several forms following the pretreatment. Hydrothermal pretreatment that employs high temperatures and pressures is gaining momentum for organics recovery from LCB which can attain value-addition. Diverse bioprocesses such as dark fermentation, anaerobic digestion etc. can be utilized following the pretreatment of LCB which can result in biohydrogen and biomethane production. Besides, integration approaches for LCB utilization that enhance process efficiency and additional products such as biohythane production as well as application of solid residue obtained after LCB pretreatment were discussed. Importance of hydrothermal pretreatment as one of the suitable strategies for LCB utilization is emphasized suggesting its future potential in large scale energy recovery.

Electrochemical-based approaches for the treatment of forever chemicals: Removal of perfluoroalkyl and polyfluoroalkyl substances (PFAS) from wastewater.

Electrochemical based approaches for the treatment of recalcitrant water borne pollutants are known to exhibit superior function in terms of efficiency and rate of treatment. Considering the stability of Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are designated as forever chemicals, which generating from various industrial activities. PFAS are contaminating the environment in small concentrations, yet exhibit severe environmental and health impacts. Electro-oxidation (EO) is a recent development that treats PFAS, in which different reactive species generates at anode due to oxidative reaction and reductive reactions at the cathode. Compared to water and wastewater treatment methods those being implemented, electrochemical approaches demonstrate superior function against PFAS. EO completely mineralizes (almost 100 %) non-biodegradable organic matter and eliminate some of the inorganic species, which proven as a robust and versatile technology. Electrode materials, electrolyte concentration pH and the current density applying for electrochemical processes determine the treatment efficiency. EO along with electrocoagulation (EC) treats PFAS along with other pollutants from variety of industries showed highest degradation of 7.69 mmol/g of PFAS. Integrated approach with other processes was found to exhibit improved efficiency in treating PFAS using several electrodes boron-doped diamond (BDD), zinc, titanium and lead based with efficiency the range of 64 to 97 %.

Application of bioelectrochemical systems to regulate and accelerate the anaerobic digestion processes.

Anaerobic digestion (AD) serves as a potential bioconversion process to treat various organic wastes/wastewaters, including sewage sludge, and generate renewable green energy. Despite its efficiency, AD has several limitations that need to be overcome to achieve maximum energy recovery from organic materials while regulating inhibitory substances. Hence, bioelectrochemical systems (BESs) have been widely investigated to treat inhibitory compounds including ammonia in AD processes and improve the AD operational efficiency, stability, and economic viability with various integrations. The BES operations as a pretreatment process, inside AD or after the AD process aids in the upgradation of biogas (CO2 to methane) and residual volatile fatty acids (VFAs) to valuable chemicals and fuels (alcohols) and even directly to electricity generation. This review presents a comprehensive summary of BES technologies and operations for overcoming the limitations of AD in lab-scale applications and suggests upscaling and future opportunities for BES-AD systems.

Enhanced bioelectrochemical treatment of petroleum refinery wastewater with Labaneh whey as co-substrate.

Petroleum refinery wastewater (PRW) that contains recalcitrant components as the major portion of constituents is difficult to treat by conventional biological processes. Microbial fuel cells (MFCs) which also produce renewable energy were found to be promising for the treatment of PRW. However, due to the high total dissolved solids and low organic matter content, the efficiency of the process is limited. Labaneh whey (LW) wastewater, having higher biodegradability and high organic matter was evaluated as co-substrate along with PRW in standard dual chambered MFC to achieve improved power generation and treatment efficiency. Among several concentrations of LW as co-substrate in the range of 5-30% (v/v) with PRW, 85:15 (PRW:LW) showed to have the highest power generation (power density (PD), 832 mW/m2), which is two times higher than the control with PRW as sole substrate (PD, 420 mW/m2). On the contrary, a maximum substrate degradation rate of 0.420 kg COD/m3-day (ξCOD, 63.10%), was registered with 80:20 feed. Higher LW ratios in PRW lead to the production of VFA which in turn gradually decreased the anolyte pH to below 4.5 (70:30 feed). This resulted in a drop in the performance of MFC with respect to power generation (274 mW/m2, 70:30 feed) and substrate degradation (ξCOD, 17.84%).

Sewage enhanced bioelectrochemical degradation of petroleum hydrocarbons in soil environment through bioelectro-stimulation.

The impact of readily biodegradable substrates (sewage and acetate) in bioelectroremediation of hydrocarbons (PW) was evaluated in a bench-scale soil-based hybrid bioelectrochemical system. Addition of bioelectro-stimulants evidenced efficient degradation than control operation. Acetate and sewage were exhibited power density of 1126 mW/m2 and 1145 mW/m2, respectively, which is almost 15 % higher than control (without stimulant, 974 mW/m2). Increased electrochemical activity was correlated well with total petroleum hydrocarbons (TPH) degradation through addition of acetate (TPHR, 525 mg/L, 67.4 %) and sewage (TPHR, 560 mg/L,71.8 %) compared to the control operation (TPHR, 503 mg/L, 64.5 %). Similarly, chemical oxygen demand (COD) reduction was also enhanced from 69.0 % (control) to 72.1 % and 74.6 % with acetate and sewage, respectively. Sewage and acetate also showed a positive role in sulfates removal, which enhanced from 56.0 % (control) to 62.9 % (acetate) and 72.6 % (sewage). This study signifies the superior function of sewage as biostimulant compared to acetate for the bioelectroremediation of hydrocarbons in contaminated soils.

Review on the production of medium and small chain fatty acids through waste valorization and CO2 fixation.

The developing approaches in the recovery of resources from biowastes for the production of renewable value-added products and fuels, using microbial cultures as bio-catalyst have now became promising aspect. In the path of anaerobic digestion, the microorganisms are assisting transformation of a complex organic feedstock/waste to biomass and biogas. This potentiality consequently leads to the production of intermediate precursors of renewable value-added products. Particularly, a set of anaerobic pathways in the fermentation process, yields small-chain fatty acids (SCFA), and medium-chain fatty acids (MCFA) via chain elongation pathways from waste valorization and CO2 fixation. This review focuses on the production of SCFA and MCFA from CO2, synthetic substrates and waste materials. Moreover, the review introduces the metabolic engineering of Escherichia coli and Saccharomyces cerevisiae for SCFAs/MCFAs production. Furtherly, it concludes that future critical research might target progress of this promising approach as a valorization of complex organic wastes.

Microbial electrosynthesis feasibility evaluation at high bicarbonate concentrations with enriched homoacetogenic biocathode.

An enrichment methodology was developed for a homoacetogenic biocathode that is able to function at high concentrations of bicarbonates for the microbial electrosynthesis (MES) of acetate from carbon dioxide. The study was performed in two stages; enrichment of consortia in serum bottles and the development of a biocathode in MES. A homoacetogenic consortium was sequentially grown under increasing concentrations of bicarbonate, in serum bottles, at room temperature. The acetate production rate was found to increase with the increase in the bicarbonate concentration and evidenced a maximum production rate of 260 mg/L d-1 (15 g HCO3-/L). On the contrary, carbon conversion efficiency decreased with the increase in the bicarbonate concentration, which evidenced a maximum at 2.5 g HCO3-/L (90.16%). Following a further increase in the bicarbonate concentration up to 20 g HCO3-/L, a visible inhibition was registered with respect to the acetate production rate and the carbon conversion efficiency. Well adapted biomass from 15 g HCO3-/L was used to develop biocathodic catalyst for MES. An effective biocathode was developed after 4 cycles of operation, during which acetate production was improved gradually, evidencing a maximum production rate of 24.53 mg acetate L-1 d-1 (carbon conversion efficiency, 47.72%). Compared to the enrichment stage, the carbon conversion efficiency and the rate of acetate production in MES were found to be low. The production of acetate induced a change in the catholyte pH, from neutral conditions towards acidic conditions.

Exploitation of Citrus Peel Extract as a Feedstock for Power Generation in Microbial Fuel Cell (MFC).

Microbial fuel cells (MFCs) are envisioned as an evolving cost-effective process for treating organic wastes to simultaneously generate bioelectricity. Therefore, in present study a single chambered mediator- less air cathode MFC was operated for bioelectricity generation using citrus waste (CW) as a feedstock. The MFC was operated at four organic loading conditions (OLs; 3, 6, 9 and 12 kg/m3). The voltage generation and organic content reduction demonstrated the possibility of utilizing CW as a substrate in MFC. The polarization analysis revealed a high-power generation of 71.1 mW/m2 with low OL of 3 kg/m3. The decrease in pH and high volatile fatty acids (VFAs) generation was noted at high OL. Our current findings suggest better performance of MFC, in terms of energy generation and organic reduction at high OL.

Utilization of residual organics of Labaneh whey for renewable energy generation through bioelectrochemical processes: Strategies for enhanced substrate conversion and energy generation.

Labaneh whey (LW) that is rich in residual organics was evaluated for bioelectricity generation using microbial fuel cell (MFC) in two different configurations namely single chamber (MFC-SC) and dual chamber (MFC-DC) MFCs. The whole study was executed in three stages: The first stage evidenced promising amount of bioelectricity generation (DC, 643 mV; SC, 545 mV) along with chemical oxygen demand removal (CODr: DC, 60.63%; SC, CODr: 55.25%). In the second phase, activity of anodic electrogenic microbes was improved with short time poising at potentials of 400, 600 and 800 mV, among which 800 mV evidenced 2.24 (DC) and 1.60 (SC) fold enhancement in power generation along with significant improvement in substrate degradation. The third phase was solely focused on bioelectrochemical treatment of LW through applied potentials for extended period. This phase achieved 89 and 94% chemical oxygen demand (COD) degradation using SC and DC configurations, respectively at 800 mV.

Removal of petroleum hydrocarbons and sulfates from produced water using different bioelectrochemical reactor configurations.

Produced water (PW) is a wastewater generated in large quantities from the extraction of oil and gas. PW found to have high amounts of dissolved solids (TDS) and residual petroleum hydrocarbons causing considerable damage to the environment. PW also contains sulfates in significant amounts, due to which treating this wastewater is essential prior to discharge. The present study was aimed for bioelectrochemical treatment of PW and simultaneous bioelectrogenesis in the two most studied configurations viz., single and dual chamber microbial fuel cells (MFCs). The study evidenced treatment of recalcitrant pollutants of PW. Both MFCs were operated by keeping similar operating conditions such as anode chamber volume, hydraulic retention time (HRT) for batch mode of operation, electrode materials, inlet characteristics of PW and ambient temperature. Among both configurations, dual chamber MFC showed higher efficiency with respect to bioelectrogenesis (single chamber - 789 mW/m2; dual chamber - 1089 mW/m2), sulfates removal (single chamber - 79.6%; dual chamber - 93.9%), total petroleum hydrocarbons removal (TPH, single chamber - 47.6%; dual chamber - 53.1%) and chemical oxygen demand degradation (COD, single chamber - 0.30 kg COD/m3-day (COD removal efficiency, 54.7%); dual chamber - 0.33 kg COD/m3-day (COD removal efficiency, 60.2%)). Evaluated polarization behavior of both MFCs were also evidenced the effective response of the electroactive anodic biofilm.

Bioelectrogenesis from Raw Algal Biomass Through Microbial Fuel Cells: Effect of Acetate as Co-substrate.

Algae are autotrophic organisms that are widespread in water bodies. Increased pollution in water bodies leads to eutrophication. However, algae growing in lakes undergoing eutrophication could be utilized towards the generation of added-value bio-electricity using microbial fuel cells (MFCs). In the present study, two methods of electricity generation using raw algae (RA) and RA + acetate (AC) as co-substrate were analyzed in single chamber air cathode MFCs. MFCs supplemented with RA and RA + AC clearly showed higher power density, greater current generation, and improved COD (chemical oxygen demand) removal, which demonstrated the feasibility of using AC as substrate for MFC. The MFC-RA + AC (0.48 mA) generated 28% higher current relative to that generated by MFC with RA alone. Notably, the maximum power densities generated by MFC-RA and MFC-RA + AC were 230 and 410 mW/m2, respectively. MFC-RA and MFC-RA + AC exhibited TCOD (total chemical oxygen reduction) removal values of 77% and 86.6%, respectively. Despite the high influent TCOD (758 mg/l) concentration, the MFC-RA + AC exhibited an 8.5% higher COD removal relative to that of MFC-RA (525 mg/l). Our current findings demonstrated effective energy generation using algae biomass with a co-substrate.

Methane as a Substrate for Energy Generation Using Microbial Fuel Cells.

Methane (CH4) is a well-known and abundant feedstock for natural gas, and is readily available from various sources. In thermal plants, the CH4 generated from anthropogenic sources is converted into electrical energy via combustion. Microbial fuel cell (MFC) technology has proven to be an efficient strategy for the biological conversion of a many substrates, including biogas (CH4), to electricity. MFC technology uses gaseous substrate along with an enriched and selective microbial consortium. Predominantly, methanotrophs and electrochemically active Geobacter were utilized in a syntrophic association on the anode of an MFC. This review focuses on the exploitation of CH4 as a substrate for bioelectrogenesis via MFCs.

A microbial fuel cell configured for the remediation of recalcitrant pollutants in soil environment.

A pristine soil environment supports a healthy soil biodiversity, which is often polluted with recalcitrant compounds. The bioelectrochemical remediation of the contaminated soils using bioelectrochemical systems (BESs) is gaining significant attention with respect to the restoration of the soil ecosystem. In this direction, a microbial fuel cell (MFC, an application of BES), was employed for the treatment of total petroleum hydrocarbons (TPHs) in a soil microenvironment at three ranges of pollution (loading conditions - 320, 590 and 840 mg TPH per L). TPHs degraded effectively in the soil-electrode vicinity in the range of 158 mg TPHR per L (320 mg TPH per L) and 356 mg TPHR per L (840 mg TPH per L). The study also demosntrated a maximum bioelectrogenesis of 286.7 mW m-2 (448 mV at 100 Ω) at the highest TPH loading concentration studied (840 mg TPH per L). The conditions prevailing in the soil MFC also facilitated the removal of sulfates (114 mg SO4 2- per L; 62.64%) and the removal of total dissolved solids (910 mg TDS per L, 12.08%) at an 840 mg TPH per L loading condition. The pH of the outlet wastewater prevailing in the mild alkaline range of 7.6 and 8.4, along with improved sulfate and TPH removal in the respective conditions suggested suitable conditions for sulfate-reducing bacteria (SRB). This study also signified the sustainability of the process for the effective treatment of hydrocarbon contaminated soil that also generates green energy.